14626-1-AP

antibody from Invitrogen Antibodies

Targeting: SHARPIN DKFZP434N1923, SIPL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14626-1-AP

Provider

Invitrogen Antibodies

Product name

SHARPIN Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAPPAGGAA AAASDLGSAA VLLAVHAAVR PLGAGPDAEA QLRRLQLSAD PERPGRFRLE LLGAGPGAVN LEWPLESVSY TIRGPTQHEL QPPPGGPGTL SLHFLNPQEA QRWAVLVRGA TVEGQNGSKS NSPPALGPEA CPVSLPSPPE ASTLKGPPPE ADLPRSPGNL TEREELAGSL ARAIAGGDEK GAAQVAAVLA QHRVALSVQL QEACFPPGPI RLQVTLEDAA SAASAASSAH VALQVHPHCT VAALQEQVFS ELGFPPAVQR WVIGRCLCVP ERSLASYGVR QDGDPAFLYL LSAPREAPAQ LVLSFLHLHQ CPRPPWL (1-326 aa encoded by BC025244)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 14626-1-AP ( SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SHARPIN antibody (Product # 14626-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14626-1-AP ( SHARPIN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 14626-1-AP ( SHARPIN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.