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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-92386 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92386, RRID:AB_11027440
- Product name
- Rabbit Polyclonal SHARPIN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SHARPIN antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHARPIN Antibody [NBP1-92386] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SHARPIN Antibody [NBP1-92386] - Analysis in human cell lines PC-3 and Caco-2 using anti-SHARPIN antibody. Corresponding SHARPIN RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
