Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- R1130P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1130P, RRID:AB_1003900
- Product name
- anti Interleukin-1 beta / IL-1B
- Antibody type
- Polyclonal
- Antigen
- The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant 153 aa human IL1 beta produced in E.coli.
- Reactivity
- Human, Canine, Simian
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 2.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot using R1130P IL1B antibody at 1/200 dilution incubated 1 h at RT to detect Dog IL-1B.The Molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1/3,000 dilution of IRDyeâ¢800 conjugated Donkey anti-Rabbit IgG for 1h at RT. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.Lane 1: Loaded with 2.5 µg of Dog IL-1B.Lane 1: Loaded with 500 ng of Dog IL-1B.