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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AAR-004-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenosine A3 Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.7 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Malme-3M cell lysate: - 1. Anti-AdenosineA3Receptor Antibody (#AAR-004), (1:200). 2. Anti-Adenosine A3Receptor Antibody , preincubated with Adenosine A3 Receptor Blocking Peptide (#BLP-AR004).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Malme-3M cell lysate: - 1. Anti-AdenosineA3Receptor Antibody (#AAR-004), (1:200). 2. Anti-Adenosine A3Receptor Antibody , preincubated with Adenosine A3 Receptor Blocking Peptide (#BLP-AR004).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of AdenosineA3Receptor in a human melanoma cell line - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human melanoma cells (A2058). A, D. Cells were stained using Anti-AdenosineA3Receptor Antibody (#AAR-004), (1:100), followed by goat Anti-rabbit AlexaFluor-555 secondary Antibody . B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged image of panels A and B. F. Merged image of panels D and E.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of AdenosineA3Receptor in rat lung - Immunohistochemical staining of paraffin-embeddedrat lung sections using Anti-AdenosineA3Receptor Antibody (#AAR-004), (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (arrows) and pneumonocytes in the alveolar wall (a). Hematoxilin is used as the counterstain.
