Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NLS689 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NLS689, RRID:AB_2289393
- Product name
- Rabbit Polyclonal Adenosine A3 R Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Human ADORA3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Reactivity
- Human, Mouse, Canine, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 0.6 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates endothelial cell activation.
CD73 is associated with poor prognosis in HNSCC.
Kutryb-Zajac B, Mierzejewska P, Sucajtys-Szulc E, Bulinska A, Zabielska MA, Jablonska P, Serocki M, Koszalka P, Milczarek R, Jasztal A, Bartoszewski R, Chlopicki S, Slominska EM, Smolenski RT
Journal of molecular and cellular cardiology 2019 Mar;128:62-76
Journal of molecular and cellular cardiology 2019 Mar;128:62-76
CD73 is associated with poor prognosis in HNSCC.
Ren ZH, Lin CZ, Cao W, Yang R, Lu W, Liu ZQ, Chen YM, Yang X, Tian Z, Wang LZ, Li J, Wang X, Chen WT, Ji T, Zhang CP
Oncotarget 2016 Sep 20;7(38):61690-61702
Oncotarget 2016 Sep 20;7(38):61690-61702
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Adenosine A3 R Antibody [NLS689] - EGFR and adenosine signaling pathways are involved in CD73-dependent EMT in HNSCC cells (A) CAL27 cells were treated with siRNA targeting CD73 or control siRNA followed by incubation with MRS3588 for another 36 h. E-cadherin, N-cadherin, Vimentin and Slug levels were evaluated. GAPDH was the internal standard for protein loading. One-way ANOVA with post-Dunnett analysis was performed using GraphPad Prism5.*P < 0.05, versus the si-control group, #P < 0.05, versus the CD73-si group. (n = 3). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/article/11435/text/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adenosine A3 R Antibody [NLS689] - Analysis of anti-Adenosine A3 Receptor / ADORA3 antibody with human tonsil, squamous epithelium at dilution 20-30 ug/ml.
