679904

antibody from BioLegend

Targeting: MMP2 CLG4, CLG4A, TBE-1

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

679904

Provider

BioLegend

Proper citation

BioLegend Cat#679904, RRID:AB_2566738

Product name

Alexa Fluor® 594 anti-MMP2

Antibody type

Monoclonal

Antigen

Recombinant human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence expressed in 293E cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

M6303D01

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

U87-MG cells were fixed with 4% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml of MMP2 (clone M6303D01) Alexa Fluor® 594 (red) in 5% FBS for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye