Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- PAB12711 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12711, RRID:AB_10553189
- Product name
- MMP2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MMP2.
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Matrix metalloproteinases MMP2 and MMP9 are produced in early stages of kidney morphogenesis but only MMP9 is required for renal organogenesis in vitro.
Lelongt B, Trugnan G, Murphy G, Ronco PM
The Journal of cell biology 1997 Mar 24;136(6):1363-73
The Journal of cell biology 1997 Mar 24;136(6):1363-73
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The protein derived from the LoVo whole cell lysate (Lane 1) or the concentrated serum free culture medium of LoVo were resolved onto 10% SDS-PAGE, transferred to NC membrane, followed by an immunoblotting with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 1000.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta stained with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)