M00811

antibody from Boster Biological Technology

Targeting: PTGS1 COX1, PGHS-1, PTGHS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00811

Provider

Boster Biological Technology

Product name

Anti-COX1 PTGS1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for COX 1/PTGS1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat COX 1/PTGS1 information: Molecular Weight: 68686 MW; Subcellular Localization: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

FHF-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of COX1 expression in A431 cell lysate (M00811). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS1 monoclonal antibody (Catalog # M00811) overnight at 4

Additional image