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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB9950 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9950, RRID:AB_1712162
- Product name
- PTGS2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant PTGS2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells.
Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins.
Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells.
Tong M, Tai HH
Biochemical and biophysical research communications 2000 Sep 16;276(1):77-81
Biochemical and biophysical research communications 2000 Sep 16;276(1):77-81
Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins.
Hittelman AB, Burakov D, Iñiguez-Lluhí JA, Freedman LP, Garabedian MJ
The EMBO journal 1999 Oct 1;18(19):5380-8
The EMBO journal 1999 Oct 1;18(19):5380-8
Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells.
Guan Z, Buckman SY, Miller BW, Springer LD, Morrison AR
The Journal of biological chemistry 1998 Oct 30;273(44):28670-6
The Journal of biological chemistry 1998 Oct 30;273(44):28670-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PTGS2 polyclonal antibody (Cat # PAB9950) is shownto detect PTGS2 present in PTGS2 transfected Sf9 cellextract (lane 1) and IL1B induced WISH cell extract (lane 2).Detection occursusing a 1 : 10,000 dilution of antibody followed by 1 : 10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately1'.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining with PTGS2 polyclonal antibody (Cat # PAB9950) was diluted 1 : 500 to detect PTGS2 in human brain cortex tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required.The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
