Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-868 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-868, RRID:AB_10000900
- Product name
- Goat Polyclonal COX-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Toll-like receptor 4 promotes high glucose-induced catabolic and inflammatory responses in chondrocytes in an NF-κB-dependent manner.
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Glucose promotes the production of interleukine-1beta and cyclooxygenase-2 in mesangial cells via enhanced (Pro)renin receptor expression.
Prolonged effects of B-type natriuretic peptide infusion on cardiac remodeling after sustained myocardial injury.
Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein.
Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein.
Liang H, Wang H, Luo L, Fan S, Zhou L, Liu Z, Yao S, Zhang X, Zhong K, Zhao H, Zha Z
Life sciences 2019 Jul 1;228:258-265
Life sciences 2019 Jul 1;228:258-265
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, Ghosh M, Saha KD, Dey S
PloS one 2014;9(5):e97599
PloS one 2014;9(5):e97599
Glucose promotes the production of interleukine-1beta and cyclooxygenase-2 in mesangial cells via enhanced (Pro)renin receptor expression.
Huang J, Siragy HM
Endocrinology 2009 Dec;150(12):5557-65
Endocrinology 2009 Dec;150(12):5557-65
Prolonged effects of B-type natriuretic peptide infusion on cardiac remodeling after sustained myocardial injury.
George I, Morrow B, Xu K, Yi GH, Holmes J, Wu EX, Li Z, Protter AA, Oz MC, Wang J
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H708-17
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H708-17
Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein.
Ballif BA, Mincek NV, Barratt JT, Wilson ML, Simmons DL
Proceedings of the National Academy of Sciences of the United States of America 1996 May 28;93(11):5544-9
Proceedings of the National Academy of Sciences of the United States of America 1996 May 28;93(11):5544-9
Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein.
Ballif BA, Mincek NV, Barratt JT, Wilson ML, Simmons DL
Proceedings of the National Academy of Sciences of the United States of America 1996 May 28;93(11):5544-9
Proceedings of the National Academy of Sciences of the United States of America 1996 May 28;93(11):5544-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX-2 Antibody [NB100-868] - A549 (A) and Daudi (B) cell lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-868] - 10x image of mouse lung tissue expressing cox-2. Secondary antibody: Alexa Fluor 546 (red), counterstain: DAPI (blue). Goat anti-mouse cox-2 immunofluorescence staining of paraffin embedded fixed mouse lung tissue sections. Antigen retrieval in citrate buffer (95C, 20 min) followed by permeabilization with 0.2% Triton X-100 for 20 min. Sections were blocked in 5% donkey serum for 1 hr at RT, and incubated with NB100-868 1:100 dilution in 3% donkey serum overnight at 4 C. Incubated with secondary donkey anti-goat AF546 at 1:500 dilution 1 hr at RT. Image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: COX-2 Antibody [NB100-868] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line)