Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP53500PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53500PU-N, RRID:AB_11144801
- Product name
- anti Cyclooxygenase 2 (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 370-399 amino acids from the Central region of Human Cyclooxygenase 2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Cyclooxygenase 2Â Antibody (Center) Cat.-No AP53500PU-NÂ in Hela cell line lysates (35ug/lane). This demonstrates the PTGS2 antibody detected the PTGS2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Cyclooxygenase 2 Antibody (Center) Cat.-No AP53500PU-N with A549 cell followed by Alexa Fluor®489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human uterus tissue stained with Cyclooxygenase 2 Antibody (Center) Cat.-No AP53500PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining