H00023429-M08

antibody from Abnova Corporation

Targeting: RYBP AAP1, DEDAF, YEAF1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023429-M08

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023429-M08, RRID:AB_1580326

Product name

RYBP monoclonal antibody (M08), clone 1F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant RYBP.

Antigen sequence

MTMGDKKSPTRPKRQAKPATDEGFWDCSVCTFRNS
AEAFKCSICDVRKGTSTRKPRINSQLVAQQVAQQY
ATPPPPKKEKKEKVEKQDKEKPEKDKEISPSVTKK
NTNKKTKPKSDILKDPPSEANSIQSANATTKTSET
NHTSRPRLKNVDRSTAQQLAVTVGNVTVIITDFKE
KTRSSSTSSSTVTSSAGSEQQNQSSSGSESTDKGS
SRSSTPKGDMSAVNDESF


Isotype

IgG

Antibody clone number

1F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4.Lane 1: RYBP transfected lysate(24.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol