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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00023429-M08 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023429-M08, RRID:AB_1214947
- Product name
- Mouse Monoclonal RYBP/DEDAF Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RYBP - RING1 and YY1 binding protein (1F4)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP expression in transfected 293T cell line by RYBP monoclonal antibody (M08), clone 1F4. Lane 1: RYBP transfected lysatE (24.8 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Detection limit for recombinant GST tagged RYBP is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RYBP/DEDAF Antibody (1F4) [H00023429-M08] - Analysis of RYBP transfected lysate using anti-RYBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RYBP rabbit polyclonal antibody.
