Explore
Validate
LearnMA5-23942
antibody from Invitrogen Antibodies
Targeting: BRCA2
BRCC2, FACD, FAD, FAD1, FANCD, FANCD1, XRCC11
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-23942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRCA2 Monoclonal Antibody (234403)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects endogenous human BRCA2.
- Antibody clone number
- 234403
- Concentration
- 0.5 mg/mL
Submitted references Loss of MAP3K7 Sensitizes Prostate Cancer Cells to CDK1/2 Inhibition and DNA Damage by Disrupting Homologous Recombination.
Washino S, Rider LC, Romero L, Jillson LK, Affandi T, Ohm AM, Lam ET, Reyland ME, Costello JC, Cramer SD
Molecular cancer research : MCR 2019 Oct;17(10):1985-1998
Molecular cancer research : MCR 2019 Oct;17(10):1985-1998
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Capan-1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of human BRCA2 Monoclonal Antibody (Product # MA5-23942) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRCA2 in Human Breast Cancer Tissue. BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using BRCA2 Monoclonal Antibody (Product # MA5-23942) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG HRP Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in epithelial cells.
