- GTX109242 - GeneTex ARG1 antibody

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Increased number of arginase 1-positive cells in the stroma of carcinomas compared to precursor lesions and nonneoplastic tissues.

Jang TJ, Kim SA, Kim MK
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Langerhans, plasmacytoid dendritic and myeloid-derived suppressor cell levels in mycosis fungoides vary according to the stage of the disease.

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Galectin-1 Reduces Neuroinflammation via Modulation of Nitric Oxide-Arginase Signaling in HIV-1 Transfected Microglia: a Gold Nanoparticle-Galectin-1 "Nanoplex" a Possible Neurotherapeutic?

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Hepatocyte-secreted extracellular vesicles modify blood metabolome and endothelial function by an arginase-dependent mechanism.

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 42263

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE GTX109242 diluted at 1:50000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody detects ARG1 protein by Western blot analysis.A. 30 µg HepG2 whole cell lysate/extract10 % SDS-PAGEArginase 1 antibody (GTX109242) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody detects Arginase 1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody detects Arginase 1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) at a dilution of 1:1000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody detects Arginase 1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I (GTX109242) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 ?g of Arginase 1 antibody (GTX109242).Western blot analysis was performed using Arginase 1 antibody (GTX109242).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 £gg of Arginase 1 antibody (GTX109242).Western blot analysis was performed using Arginase 1 antibody (GTX109242).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I (GTX109242) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Arginase 1 antibody (GTX109242) diluted at 1:2500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: Arginase 1 antibody (GTX109242) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX109242

Antibody data