OAGA00704

antibody from Aviva Systems Biology

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAGA00704

Provider

Aviva Systems Biology

Proper citation

Aviva Systems Biology Cat#OAGA00704, RRID:AB_2630636

Product name

TJP1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Sample(30 ug whole cell lysate)A:H12995% SDS PAGEOAGA00704 diluted at 1:3000

Immunocytochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TJP1 protein stained by ZO-1 antibody [N1N2], N-term (OAGA00704) diluted at 1:500.Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1(OAGA00704) antibody at 1:250 dilution.