PAB20077

antibody from Abnova Corporation

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20077

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20077, RRID:AB_10961736

Product name

TJP1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TJP1.

Antigen sequence

ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEI
SQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAK
TLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSA
NASERDDISEIQSLASDHSGR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with TJP1 polyclonal antibody (Cat # PAB20077) at 1-4 ug/mL dilution shows positivity in plasma membrane, cytoplasm, cell junctions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with TJP1 polyclonal antibody (Cat # PAB20077) shows distinct membranous and cytoplasmic positivity in cells of glomeruli at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)