Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA3-39100-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Canine
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- ZO1-1A12
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.
Zwitterionic micelles efficiently deliver oral insulin without opening tight junctions.
Lee CW, Kuo CC, Liang CJ, Pan HJ, Shen CN, Lee CH
BMC molecular and cell biology 2022 Jul 6;23(1):26
BMC molecular and cell biology 2022 Jul 6;23(1):26
Zwitterionic micelles efficiently deliver oral insulin without opening tight junctions.
Han X, Lu Y, Xie J, Zhang E, Zhu H, Du H, Wang K, Song B, Yang C, Shi Y, Cao Z
Nature nanotechnology 2020 Jul;15(7):605-614
Nature nanotechnology 2020 Jul;15(7):605-614
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-39100-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The TNT formation induced by macrophage CMs is correlated with enhanced cell motility and EMT. a Migration speeds of PANC-1 cells in DMEM and macrophage CMs. In each experiment, we measured the speeds of 45 cells. b Invasion in 24 h of PANC-1 cells in DMEM and macrophage CMs. c mRNA levels of vimentin (VIM) and Zeb-1 (ZEB1) in PANC-1 cells cultured in DMEM and macrophage CMs. For Zeb-1, the P -value between PANC-1 cells in DMEM and M0 CM is 0.17; while that between PANC-1 cells in DMEM and M1 CM is 0.34. d mRNA levels of connexin 43 (Cx43) in PANC-1 cells cultured in DMEM and macrophage CMs. All data were obtained from three independent experiments. ***, P < 0.005; **, P < 0.01; *, P < 0.05 (post hoc Tukey's test) in comparison with the PANC-1 cells in DMEM. e Confocal fluorescence images of ZO-1 and beta-catenin of PANC-1 cells in DMEM and macrophage CMs
- Conjugate
- Green dye