Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-24716 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZO-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Roxadustat (FG-4592) Facilitates Recovery From Renal Damage by Ameliorating Mitochondrial Dysfunction Induced by Folic Acid.
Rapid generation of sub-type, region-specific neurons and neural networks from human pluripotent stem cell-derived neurospheres.
Li X, Jiang B, Zou Y, Zhang J, Fu YY, Zhai XY
Frontiers in pharmacology 2021;12:788977
Frontiers in pharmacology 2021;12:788977
Rapid generation of sub-type, region-specific neurons and neural networks from human pluripotent stem cell-derived neurospheres.
Begum AN, Guoynes C, Cho J, Hao J, Lutfy K, Hong Y
Stem cell research 2015 Nov;15(3):731-741
Stem cell research 2015 Nov;15(3):731-741
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a ZO1 polyclonal antibody (Product # PA5-24716) in K562 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded lung carcinoma using a ZO1 polyclonal antibody (Product # PA5-24716), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of K562 cells using a ZO1 polyclonal antibody (Product # PA5-24716) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 FG-4592 pretreatment promoted the restoration of tubular structural integrity in FA-induced renal damage. (A) Renal micrographs of IHC for E-cadherin and ZO-1. (B) Western blot analysis of E-cadherin and ZO-1. (C) Relative abundance of E-cadherin. (D) Relative abundance of ZO-1. The data are expressed as the mean +- SE. Scale = 50 mum, * p < 0.05, ** p < 0.01.