PAB28487

antibody from Abnova Corporation

Targeting: TJP2 DFNA51, X104, ZO-2, ZO2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28487

Provider

Abnova Corporation

Product name

TJP2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TJP2

Antigen sequence

IGVLLMKSRANEEYGLRLGSQIFVKEMTRTGLATK
DGNLHEGDIILKINGTVTENMSLTDARKLIEKSRG
KLQLVVLRDSQQTLINIPSLNDSDSEIEDISEIES
NRSFSPEERRHQYSDYDYHSSSEKLKERPSSREDT
PSRLSRMG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with TJP2 polyclonal antibody at 1:100-1:250 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with TJP2 polyclonal antibody at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with TJP2 polyclonal antibody (Cat # PAB28487) at 1-4 ug/ml shows positivity in plasma membrane, cytoplasm & cell junctions.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with TJP2 polyclonal antibody (Cat # PAB28487) shows moderate cytoplasmic and membranous positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)