Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-86850 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86850, RRID:AB_11014979
- Product name
- Rabbit Polyclonal Tight Junction Protein 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Tight Junction Protein 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Downregulation of tight junction protein zonula occludens-2 and urothelium damage in a cyclophosphamide-induced mouse model of cystitis.
Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1β.
A promising protein responsible for overactive bladder in ovariectomized mice.
Sequential development of intercellular junctions in bioengineered human corneas.
Chen YH, Chen CJ, Wang SJ, Lin YN, Chen WC, Tsai MY, Chen HY
Taiwanese journal of obstetrics & gynecology 2018 Jun;57(3):399-406
Taiwanese journal of obstetrics & gynecology 2018 Jun;57(3):399-406
Spleen-derived classical monocytes mediate lung ischemia-reperfusion injury through IL-1β.
Hsiao HM, Fernandez R, Tanaka S, Li W, Spahn JH, Chiu S, Akbarpour M, Ruiz-Perez D, Wu Q, Turam C, Scozzi D, Takahashi T, Luehmann HP, Puri V, Budinger GRS, Krupnick AS, Misharin AV, Lavine KJ, Liu Y, Gelman AE, Bharat A, Kreisel D
The Journal of clinical investigation 2018 Jul 2;128(7):2833-2847
The Journal of clinical investigation 2018 Jul 2;128(7):2833-2847
A promising protein responsible for overactive bladder in ovariectomized mice.
Chen HY, Chen CJ, Chen WC, Wang SJ, Chen YH
Taiwanese journal of obstetrics & gynecology 2017 Apr;56(2):196-203
Taiwanese journal of obstetrics & gynecology 2017 Apr;56(2):196-203
Sequential development of intercellular junctions in bioengineered human corneas.
González-Andrades M, Garzón I, Gascón MI, Muñoz-Avila JI, Sánchez-Quevedo MC, Campos A, Alaminos M
Journal of tissue engineering and regenerative medicine 2009 Aug;3(6):442-9
Journal of tissue engineering and regenerative medicine 2009 Aug;3(6):442-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Primary mouse lung endothelial cells treated with or without control IgG was blotted with the anti-TJP-2 antibody. Primary Antibody diluted 1:1000. Image submitted by a verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tight Junction Protein 2 Antibody [NBP1-86850] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-TJP2 antibody. Corresponding TJP2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human testis shows strong membranous and cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human colon shows moderate cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human cerebral cortex shows strong cytoplasmic psotivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Tight Junction Protein 2 Antibody [NBP1-86850] - Staining of human pancreas shows weak cytoplasmic psotivity in exocrine glandular cells.