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- Antibody Data
- Antigen structure
- References [34]
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- Immunohistochemistry [2]
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- Product number
- NB100-1992 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1992, RRID:AB_2239544
- Product name
- Rabbit Polyclonal SOD2/Mn-SOD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects 25kDa protein, corresponding to the molecular mass of Mn superoxide dismutase (SOD) on SDS-PAGE immunoblots.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Drosophila, Goat, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction.
Melatonin reverses the oxidative stress and mitochondrial dysfunction caused by LETM1 silencing.
The Polycomb Protein Bmi1 Plays a Crucial Role in the Prevention of 1,25(OH)2 D Deficiency-Induced Bone Loss.
Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence.
5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina.
Effects of nitric oxide on steroidogenesis and apoptosis in goat luteinized granulosa cells.
Dietary N-carbamylglutamate and l-arginine supplementation improves intestinal energy status in intrauterine-growth-retarded suckling lambs.
Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.
Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-κB and NLRP3 inflammasome.
Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.
Effects of nutrient restriction and arginine treatment on oxidative stress in the ovarian tissue of ewes during the luteal phase.
Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells.
H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes.
In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet.
Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia.
The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication.
Genetic and pharmacological evidence implicates cathepsins in Niemann-Pick C cerebellar degeneration.
Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits.
Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs.
The kinase Btk negatively regulates the production of reactive oxygen species and stimulation-induced apoptosis in human neutrophils.
Shc proteins influence the activities of enzymes involved in fatty acid oxidation and ketogenesis.
Reduction of oxidative stress, amyloid deposition, and memory deficit by manganese superoxide dismutase overexpression in a transgenic mouse model of Alzheimer's disease.
Posttranslational modifications in Cu,Zn-superoxide dismutase and mutations associated with amyotrophic lateral sclerosis.
Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3.
An immunohistochemical study of copper/zinc superoxide dismutase and manganese superoxide dismutase in rat hippocampus after transient cerebral ischemia.
An immunohistochemical study of copper/zinc superoxide dismutase and manganese superoxide dismutase in rat hippocampus after transient cerebral ischemia.
Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody.
Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody.
Synthesis and processing of the precursor for human mangano-superoxide dismutase.
Synthesis and processing of the precursor for human mangano-superoxide dismutase.
Biosynthesis and regulation of superoxide dismutases.
Biosynthesis and regulation of superoxide dismutases.
Aspects of the structure, function, and applications of superoxide dismutase.
Aspects of the structure, function, and applications of superoxide dismutase.
Zhang H, Ma Y, Wang M, Elsabagh M, Loor JJ, Wang H
Food & function 2020 May 1;11(5):4456-4470
Food & function 2020 May 1;11(5):4456-4470
Melatonin reverses the oxidative stress and mitochondrial dysfunction caused by LETM1 silencing.
Aral C, Demirkesen S, Bircan R, Yasar Sirin D
Cell biology international 2020 Mar;44(3):795-807
Cell biology international 2020 Mar;44(3):795-807
The Polycomb Protein Bmi1 Plays a Crucial Role in the Prevention of 1,25(OH)2 D Deficiency-Induced Bone Loss.
Sun H, Qiao W, Cui M, Yang C, Wang R, Goltzman D, Jin J, Miao D
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Mar;35(3):583-595
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Mar;35(3):583-595
Pyrroloquinoline Quinone Prevents Estrogen Deficiency-Induced Osteoporosis by Inhibiting Oxidative Stress and Osteocyte Senescence.
Geng Q, Gao H, Yang R, Guo K, Miao D
International journal of biological sciences 2019;15(1):58-68
International journal of biological sciences 2019;15(1):58-68
5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina.
Xie MY, Yang Y, Liu P, Luo Y, Tang SB
International journal of ophthalmology 2019;12(1):1-7
International journal of ophthalmology 2019;12(1):1-7
Effects of nitric oxide on steroidogenesis and apoptosis in goat luteinized granulosa cells.
Guo YX, Zhang GM, Yao XL, Tong R, Cheng CY, Zhang TT, Wang ST, Yang H, Wang F
Theriogenology 2019 Mar 1;126:55-62
Theriogenology 2019 Mar 1;126:55-62
Dietary N-carbamylglutamate and l-arginine supplementation improves intestinal energy status in intrauterine-growth-retarded suckling lambs.
Zhang H, Peng A, Guo S, Wang M, Loor JJ, Wang H
Food & function 2019 Apr 17;10(4):1903-1914
Food & function 2019 Apr 17;10(4):1903-1914
Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism.
Fauzia E, Barbhuyan TK, Shrivastava AK, Kumar M, Garg P, Khan MA, Robertson AAB, Raza SS
Frontiers in pharmacology 2018;9:1034
Frontiers in pharmacology 2018;9:1034
Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-κB and NLRP3 inflammasome.
Du Y, Gu X, Meng H, Aa N, Liu S, Peng C, Ge Y, Yang Z
American journal of translational research 2018;10(12):4235-4246
American journal of translational research 2018;10(12):4235-4246
Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.
Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, Bernhard O, Balleiniger M, Alfar EA, Zieseniss A, Toischer K, Guan K, Rizzoli SO, Brügger B, Fischer A, Katschinski DM, Rehling P, Dudek J
Cell reports 2018 Oct 16;25(3):561-570.e6
Cell reports 2018 Oct 16;25(3):561-570.e6
Effects of nutrient restriction and arginine treatment on oxidative stress in the ovarian tissue of ewes during the luteal phase.
Guo YX, Nie HT, Xu CJ, Zhang GM, Sun LW, Zhang TT, Wang Z, Feng X, You PH, Wang F
Theriogenology 2018 Jun;113:127-136
Theriogenology 2018 Jun;113:127-136
Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells.
Yao X, Zhang G, Guo Y, Ei-Samahy M, Wang S, Wan Y, Han L, Liu Z, Wang F, Zhang Y
Theriogenology 2017 Oct 15;102:162-173
Theriogenology 2017 Oct 15;102:162-173
H2O2 induces caveolin‑1 degradation and impaired mitochondrial function in E11 podocytes.
Chen YH, Lin WW, Liu CS, Su SL
Molecular medicine reports 2017 Nov;16(5):7841-7847
Molecular medicine reports 2017 Nov;16(5):7841-7847
In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet.
Freitas I, Boncompagni E, Tarantola E, Gruppi C, Bertone V, Ferrigno A, Milanesi G, Vaccarone R, Tira ME, Vairetti M
Oxidative medicine and cellular longevity 2016;2016:9307064
Oxidative medicine and cellular longevity 2016;2016:9307064
Caveolin-1 Expression Ameliorates Nephrotic Damage in a Rabbit Model of Cholesterol-Induced Hypercholesterolemia.
Chen YH, Lin WW, Liu CS, Hsu LS, Lin YM, Su SL
PloS one 2016;11(4):e0154210
PloS one 2016;11(4):e0154210
The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication.
Zhang Y, Chen Y, Gucek M, Xu H
The EMBO journal 2016 May 17;35(10):1045-57
The EMBO journal 2016 May 17;35(10):1045-57
Genetic and pharmacological evidence implicates cathepsins in Niemann-Pick C cerebellar degeneration.
Chung C, Puthanveetil P, Ory DS, Lieberman AP
Human molecular genetics 2016 Apr 1;25(7):1434-46
Human molecular genetics 2016 Apr 1;25(7):1434-46
Caveolin-1 provides palliation for adverse hepatic reactions in hypercholesterolemic rabbits.
Chen YH, Lin WW, Liu CS, Hsu LS, Lin YM, Su SL
PloS one 2014;9(1):e71862
PloS one 2014;9(1):e71862
Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs.
Chan JK, Charrier JG, Kodani SD, Vogel CF, Kado SY, Anderson DS, Anastasio C, Van Winkle LS
Particle and fibre toxicology 2013 Aug 1;10:34
Particle and fibre toxicology 2013 Aug 1;10:34
The kinase Btk negatively regulates the production of reactive oxygen species and stimulation-induced apoptosis in human neutrophils.
Honda F, Kano H, Kanegane H, Nonoyama S, Kim ES, Lee SK, Takagi M, Mizutani S, Morio T
Nature immunology 2012 Feb 26;13(4):369-78
Nature immunology 2012 Feb 26;13(4):369-78
Shc proteins influence the activities of enzymes involved in fatty acid oxidation and ketogenesis.
Hagopian K, Tomilov AA, Tomilova N, Kim K, Taylor SL, Lam AK, Cortopassi GA, McDonald RB, Ramsey JJ
Metabolism: clinical and experimental 2012 Dec;61(12):1703-13
Metabolism: clinical and experimental 2012 Dec;61(12):1703-13
Reduction of oxidative stress, amyloid deposition, and memory deficit by manganese superoxide dismutase overexpression in a transgenic mouse model of Alzheimer's disease.
Dumont M, Wille E, Stack C, Calingasan NY, Beal MF, Lin MT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Aug;23(8):2459-66
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Aug;23(8):2459-66
Posttranslational modifications in Cu,Zn-superoxide dismutase and mutations associated with amyotrophic lateral sclerosis.
Furukawa Y, O'Halloran TV
Antioxidants & redox signaling 2006 May-Jun;8(5-6):847-67
Antioxidants & redox signaling 2006 May-Jun;8(5-6):847-67
Synthesis and anti-inflammatory activity of a chimeric recombinant superoxide dismutase: SOD2/3.
Gao B, Flores SC, Leff JA, Bose SK, McCord JM
American journal of physiology. Lung cellular and molecular physiology 2003 Jun;284(6):L917-25
American journal of physiology. Lung cellular and molecular physiology 2003 Jun;284(6):L917-25
An immunohistochemical study of copper/zinc superoxide dismutase and manganese superoxide dismutase in rat hippocampus after transient cerebral ischemia.
Liu XH, Kato H, Nakata N, Kogure K, Kato K
Brain research 1993 Oct 15;625(1):29-37
Brain research 1993 Oct 15;625(1):29-37
An immunohistochemical study of copper/zinc superoxide dismutase and manganese superoxide dismutase in rat hippocampus after transient cerebral ischemia.
Liu XH, Kato H, Nakata N, Kogure K, Kato K
Brain research 1993 Oct 15;625(1):29-37
Brain research 1993 Oct 15;625(1):29-37
Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody.
Adachi T, Ohta H, Yamada H, Futenma A, Kato K, Hirano K
Clinica chimica acta; international journal of clinical chemistry 1992 Nov 30;212(3):89-102
Clinica chimica acta; international journal of clinical chemistry 1992 Nov 30;212(3):89-102
Quantitative analysis of extracellular-superoxide dismutase in serum and urine by ELISA with monoclonal antibody.
Adachi T, Ohta H, Yamada H, Futenma A, Kato K, Hirano K
Clinica chimica acta; international journal of clinical chemistry 1992 Nov 30;212(3):89-102
Clinica chimica acta; international journal of clinical chemistry 1992 Nov 30;212(3):89-102
Synthesis and processing of the precursor for human mangano-superoxide dismutase.
Wispé JR, Clark JC, Burhans MS, Kropp KE, Korfhagen TR, Whitsett JA
Biochimica et biophysica acta 1989 Jan 19;994(1):30-6
Biochimica et biophysica acta 1989 Jan 19;994(1):30-6
Synthesis and processing of the precursor for human mangano-superoxide dismutase.
Wispé JR, Clark JC, Burhans MS, Kropp KE, Korfhagen TR, Whitsett JA
Biochimica et biophysica acta 1989 Jan 19;994(1):30-6
Biochimica et biophysica acta 1989 Jan 19;994(1):30-6
Biosynthesis and regulation of superoxide dismutases.
Hassan HM
Free radical biology & medicine 1988;5(5-6):377-85
Free radical biology & medicine 1988;5(5-6):377-85
Biosynthesis and regulation of superoxide dismutases.
Hassan HM
Free radical biology & medicine 1988;5(5-6):377-85
Free radical biology & medicine 1988;5(5-6):377-85
Aspects of the structure, function, and applications of superoxide dismutase.
Bannister JV, Bannister WH, Rotilio G
CRC critical reviews in biochemistry 1987;22(2):111-80
CRC critical reviews in biochemistry 1987;22(2):111-80
Aspects of the structure, function, and applications of superoxide dismutase.
Bannister JV, Bannister WH, Rotilio G
CRC critical reviews in biochemistry 1987;22(2):111-80
CRC critical reviews in biochemistry 1987;22(2):111-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Superoxide Dismutase 2 Antibody [NB100-1992] - Western blot analysis of rat brain tissue extract, probed with Mn SOD polyclonal antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Superoxide Dismutase 2 Antibody [NB100-1992] - Western blot analysis of Mn SOD in rat tissue lysates, using a 1:1000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOD2/Mn-SOD Antibody [NB100-1992] - Analysis of Rat Tissue lysates showing detection of SOD2 protein using Rabbit Anti-SOD2 Polyclonal Antibody. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NB100-1992] - Superoxide Dismutase 2 Antibody [NB100-1992] - Analysis of mouse back skin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SOD2/Mn-SOD Antibody [NB100-1992] - The effects of a high calcium/phosphate diet, of 1,25(OH)2D3, and of antioxidant supplementation on oxidative stress, DNA damage, and protein expression of oncogenes and tumor suppressive genes in 1alpha(OH)ase -/- mice. Mice from each group were treated as described in Figure 1. Representative micrographs of skin sections stained immunohistochemically for SOD2 and gamma-H2AX. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/acel.12951), licensed under a CC-BY licence.
