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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- R31882 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Superoxide Dismutase 2 Antibody / SOD2
- Antibody type
- Polyclonal
- Antigen
- Amino acids QYKNVRPDYLKAIWNVINWENVTERYMACKK of human SOD2 were used as the immunogen for the SOD2 antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the SOD2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse 1) testis, 2) lung, 3) heart, 4) liver and 5) human HepG2 lysate with SOD2 antibody. Expected/observed molecular weight ~25 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human SMMC-7721 cells with SOD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human A549 cells with SOD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer with SOD2 antibdoy. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
