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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP03024PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-SOD2 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-SOD2 Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ug
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis using SOD2 Antibody at 2 ug/ml in 1 mg/ml lysate, HeLa 10 ug (Lane 1+2), Raw 20ug (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST).?R1634HRP anti-Rabbit IgG (H+L)-HRP secondary antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SOD2 Antibody on Raw 204.4 at 5 ug/ml (Left) , incubated with F(ab)2 Goat anti rabbit IgG (H+L)-FITC conjugated secondary antibody (R1410 , 1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistohemistry localization of Mn SOD within the muscle fibres of LPS-injected rats (Left: Untreated, Right: treated with 3mmol*kg-1 NAC).?Courtesy of E. Barreiro, IMIM, Spain
- Validation comment
- IHC
