MAB3419

antibody from R&D Systems

Targeting: SOD2 GClnc1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3419

Provider

R&D Systems

Product name

Human/Mouse/Rat SOD2/Mn-SOD Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat SOD2 in Western blots. In Western blots, no cross-reactivity with recombinant human SOD1 or SOD3 is observed.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P04179


Isotype

IgG

Antibody clone number

349810

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells.

Wohlrab P, Soto-Gonzales L, Benesch T, Winter MP, Lang IM, Markstaller K, Tretter V, Klein KU
Frontiers in physiology 2018;9:1754

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The AB loop of oncostatin M (OSM) determines species-specific signaling in humans and mice.

Adrian-Segarra JM, Sreenivasan K, Gajawada P, Lörchner H, Braun T, Pöling J
The Journal of biological chemistry 2018 Dec 28;293(52):20181-20199

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Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming.

Sun S, Liu J, Zhao M, Han Y, Chen P, Mo Q, Wang B, Chen G, Fang Y, Tian Y, Zhou J, Ma D, Gao Q, Wu P
Cell death & disease 2017 Jun 8;8(6):e2870

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Early Cellular Changes in the Ascending Aorta and Myocardium in a Swine Model of Metabolic Syndrome.

Saraf R, Huang T, Mahmood F, Owais K, Bardia A, Khabbaz KR, Liu D, Senthilnathan V, Lassaletta AD, Sellke F, Matyal R
PloS one 2016;11(1):e0146481

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Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress.

Van Brussel I, Schrijvers DM, Martinet W, Pintelon I, Deschacht M, Schnorbusch K, Maes L, Bosmans JM, Vrints CJ, Adriaensen D, Cos P, Bult H
PloS one 2012;7(8):e43357

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Sertraline slows disease progression and increases neurogenesis in N171-82Q mouse model of Huntington's disease.

Duan W, Peng Q, Masuda N, Ford E, Tryggestad E, Ladenheim B, Zhao M, Cadet JL, Wong J, Ross CA
Neurobiology of disease 2008 Jun;30(3):312-22

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat SOD2/Mn-SOD by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD2/Mn-SOD was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOD2/Mn-SOD in HL-60 Human Cell Line. SOD2/Mn-SOD was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.