H00006647-M04

antibody from Abnova Corporation

Targeting: SOD1 ALS, ALS1, IPOA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006647-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006647-M04, RRID:AB_10902011

Product name

SOD1 monoclonal antibody (M04), clone 10D5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant SOD1.

Antigen sequence

MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGS
IKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSR
KHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVI
SLSGDHCITGRTLVVHEKADDLGKGGNEESTKTGN
AGSRLACGVIGIAQ


Isotype

IgG

Antibody clone number

10D5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SOD1 expression in transfected 293T cell line by SOD1 monoclonal antibody (M04), clone 10D5.Lane 1: SOD1 transfected lysate (Predicted MW: 15.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SOD1 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SOD1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol