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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA500495 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500495, RRID:AB_2193906
- Product name
- SOD1 (Superoxide Dismutase 1) mouse monoclonal antibody, clone OTI8B10 (formerly 8B10)
- Antibody type
- Monoclonal
- Description
- SOD1 (Superoxide Dismutase 1) mouse monoclonal antibody, clone OTI8B10 (formerly 8B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOD1
- Isotype
- IgG
- Antibody clone number
- OTI8B10
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Pterostilbene Decreases the Antioxidant Defenses of Aggressive Cancer Cells In Vivo: A Physiological Glucocorticoids- and Nrf2-Dependent Mechanism.
Benlloch M, Obrador E, Valles SL, Rodriguez ML, Sirerol JA, Alcácer J, Pellicer JA, Salvador R, Cerdá C, Sáez GT, Estrela JM
Antioxidants & redox signaling 2016 Jun 10;24(17):974-90
Antioxidants & redox signaling 2016 Jun 10;24(17):974-90
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SOD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD1 (RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 6 different cell lines by using anti-SOD1 monoclonal antibody (HepG2: human; HeLa: human; HT29: human; SVT2: mouse; A549: human; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500495)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200725 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOD1 antibody(TA500495), and then analyzed by flow cytometry.
- Validation comment
- FC
