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- Product number
- MAB1819 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Klotho Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Klotho (aa 23-550 and aa 35-982) in direct ELISAs and Western blots. Does not cross-react with recombinant human Klotho or recombinant mouse Klotho B.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 236214
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The cooperation of FGF receptor and Klotho is involved in excretory canal development and regulation of metabolic homeostasis in Caenorhabditis elegans.
Polanska UM, Edwards E, Fernig DG, Kinnunen TK
The Journal of biological chemistry 2011 Feb 18;286(7):5657-66
The Journal of biological chemistry 2011 Feb 18;286(7):5657-66
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Mouse Klotho ELISA Standard Curve. Recombinant Mouse Klotho protein was serially diluted 2-fold and captured by Rat Anti-Mouse Klotho Monoclonal Antibody (Catalog # MAB1819) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Klotho Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1819) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
