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- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP1-76511 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76511, RRID:AB_11025265
- Product name
- Rabbit Polyclonal Klotho Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Three isoforms of KLOTHO are known to exist.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dipeptidyl peptidase-4 inhibition with linagliptin prevents western diet-induced vascular abnormalities in female mice.
Manrique C, Habibi J, Aroor AR, Sowers JR, Jia G, Hayden MR, Garro M, Martinez-Lemus LA, Ramirez-Perez FI, Klein T, Meininger GA, DeMarco VG
Cardiovascular diabetology 2016 Jul 8;15:94
Cardiovascular diabetology 2016 Jul 8;15:94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Klotho Antibody [NBP1-76511] - Analysis in HepG2 cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Klotho Antibody [NBP1-76511] - Validation in Rat Kidney Tissue Lysate Loading: 15ug of lysates per lane. Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Klotho Antibody [NBP1-76511] - via Protein Expression Profile in Human and Mouse Cell Lines Loading: 15ug of lysates per lane. Antibodies: KLOTHO 6107 (2 g/mL), KLOTHO, competitor antibody (4ug/mL) and beta-actin (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Klotho Antibody [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Klotho Antibody [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Klotho Antibody [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY licence.
