MA5-29597

antibody from Invitrogen Antibodies

Targeting: TFPI EPI, LACI, TFI, TFPI1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29597

Provider

Invitrogen Antibodies

Product name

TFPI Recombinant Rabbit Monoclonal Antibody (6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human TFPI/LACI/EPI.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

6

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TFPI in Lane A: MCF7 Whole Cell Lysate (30 µg). Samples were probed using a TFPI Monoclonal Antibody (Product # MA5-29597) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:35 kDa. Observed band size:40 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TFPI Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29597) at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 40 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TFPI Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 0.5 µL with TFPI Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29597) and 15 µL of 50 % Protein G agarose. Primary antibody: TFPI Recombinant Rabbit Monoclonal Antibody (6), at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 45 kDa.