PA5-118303

antibody from Invitrogen Antibodies

Targeting: TBCE HRD, KCS, KCS1, pac2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118303

Provider

Invitrogen Antibodies

Product name

TBCE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TBCE Polyclonal Antibody (Product # PA5-118303) at 1:500 dilution. Lane A: K562 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 55 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TBCE Polyclonal Antibody (Product # PA5-118303) at 1:500 dilution. Lane A: K562 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 55 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human TBCE in human liver with TBCE Polyclonal Antibody (Product # PA5-118303, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TBCE Immunoprecipitation using: Lane A: 0.5 mg Jurkat HeLa Whole Cell Lysate 4 µL with TBCE Polyclonal Antibody (Product # PA5-118303) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TBCE Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa.