Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77286 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-1d Adrenoceptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
Submitted references Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis.
Gao R, Peng X, Perry C, Sun H, Ntokou A, Ryu C, Gomez JL, Reeves BC, Walia A, Kaminski N, Neumark N, Ishikawa G, Black KE, Hariri LP, Moore MW, Gulati M, Homer RJ, Greif DM, Eltzschig HK, Herzog EL
The Journal of clinical investigation 2021 Jan 4;131(1)
The Journal of clinical investigation 2021 Jan 4;131(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates with alpha-1d Adrenoceptor (extracellular) polyclonal antibody (Product # PA5-77286) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of alpha-1d Adrenoceptor (extracellular) in rat neocortex. A) Samples were probed with alpha-1d Adrenoceptor (extracellular) polyclonal antibody (Product # PA5-77286) (green) and incubated with parvalbumin. B) Stains of Purkinje nerve cells. C) Confocal merged image.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of alpha-1d Adrenoceptor (extracellular) in rat neocortex. A) Samples were probed with alpha-1d Adrenoceptor (extracellular) polyclonal antibody (Product # PA5-77286) (green) and incubated with parvalbumin. B) Stains of Purkinje nerve cells. C) Confocal merged image.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using polyclonal alpha-1d Adrenoceptor (extracellular) antibody (Product # PA5-77286) with a dilution of 5-10 µg antibody/0.5x10^6 cells.