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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AAR-019-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha 1D-Adrenergic Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates: - 1,2,5,6. Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:200).3,4,7,8. Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody , preincubated with alpha 1D-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR019).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates: - 1,2,5,6. Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:200).3,4,7,8. Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody , preincubated with alpha 1D-Adrenergic Receptor (extracellular) Blocking Peptide (#BLP-AR019).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of alpha 1D-Adrenoceptorin rat PC12 cells - Cell surface detection of alpha 1D-Adrenoceptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019) (1:100)followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody . B. Merge of A and live view of the cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of alpha 1D-Adrenoceptor in rat neocortex - Immunohistochemical staining of alpha 1D-adrenoceptor in rat neocortex using Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019). A. Most intense staining of alpha 1D-adrenoceptor (green) appears in apical dendrites (thin horizontal arrows) but also in the soma (thick horizontal arrow). Few cortical interneurons express alpha 1D-adrenoceptor. B. The same section was also stained for parvalbumin and one cell (marked with a vertical arrow) also expresses alpha 1D-adrenoceptor.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of alpha 1D-Adrenoceptor in rat heart - Immunohistochemical staining of rat heart paraffin embedded sections using Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (1:100). alpha 1D-Adrenoceptor is expressed in cardiomyocytes of the myocardium and in the smooth muscle of the blood vessels (arrows). Hematoxilin is used as the counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of alpha 1D-Adrenoceptorin intact live Jurkat cells: - (black) Unstained cells. (green) Cells + Anti- alpha 1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), (5-10 µg Antibody /0.5x106 cells).
