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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF737 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat P-Selectin/CD62P Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q01102- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation.
Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin.
Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack.
Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma.
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets.
Xu G, Li C, Parsiola AL, Li J, McCarter KD, Shi R, Mayhan WG, Sun H
Frontiers in cellular neuroscience 2019;13:6
Frontiers in cellular neuroscience 2019;13:6
Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin.
Fournier AP, Quenault A, Martinez de Lizarrondo S, Gauberti M, Defer G, Vivien D, Docagne F, Macrez R
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 6;114(23):6116-6121
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 6;114(23):6116-6121
Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack.
Quenault A, Martinez de Lizarrondo S, Etard O, Gauberti M, Orset C, Haelewyn B, Segal HC, Rothwell PM, Vivien D, Touzé E, Ali C
Brain : a journal of neurology 2017 Jan;140(1):146-157
Brain : a journal of neurology 2017 Jan;140(1):146-157
Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma.
Mizrachi A, Shamay Y, Shah J, Brook S, Soong J, Rajasekhar VK, Humm JL, Healey JH, Powell SN, Baselga J, Heller DA, Haimovitz-Friedman A, Scaltriti M
Nature communications 2017 Feb 13;8:14292
Nature communications 2017 Feb 13;8:14292
Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis.
Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC Jr, Vlasuk GP, Pittman DD
Atherosclerosis 2008 Nov;201(1):53-66
Atherosclerosis 2008 Nov;201(1):53-66
Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ
Atherosclerosis 2008 Jul;199(1):55-64
Atherosclerosis 2008 Jul;199(1):55-64
A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets.
Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, McVicar DW
Blood 2004 Aug 15;104(4):1042-7
Blood 2004 Aug 15;104(4):1042-7
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat P-Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat P-Selectin/CD62P by Simple WesternTM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P-Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- P-Selectin/CD62P in Mouse Liver. P-Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
