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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN499183 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-AAK1 (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- A 20 amino acid peptide near the amino terminus of the uman Aak1 (AP30003CP-N)
- Description
- Affinity chromatography purified via peptide column
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- N-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.
The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton.
Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.
Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals.
Henderson DM, Conner SD
Molecular biology of the cell 2007 Jul;18(7):2698-706
Molecular biology of the cell 2007 Jul;18(7):2698-706
The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton.
Smythe E, Ayscough KR
EMBO reports 2003 Mar;4(3):246-51
EMBO reports 2003 Mar;4(3):246-51
Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.
Conner SD, Schmid SL
The Journal of cell biology 2002 Mar 4;156(5):921-9
The Journal of cell biology 2002 Mar 4;156(5):921-9
Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals.
Ricotta D, Conner SD, Schmid SL, von Figura K, Honing S
The Journal of cell biology 2002 Mar 4;156(5):791-5
The Journal of cell biology 2002 Mar 4;156(5):791-5
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Aak1 in A-20 lysate with AP30003PU-N Aak1 antibody at (A) 1 and (B) 2 ?g/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of Aak1 in A-20 cells with AP30003PU-N Aak1 antibody at 5 ?g/ml.
