bs-0608R

antibody from Bioss

Targeting: ICAM1 BB2, CD54

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-0608R

Provider

Bioss

Proper citation

Bioss Cat#bs-0608R, RRID:AB_10856895

Product name

Rabbit Anti-ICAM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

ICAM1

Description

Purified by Protein A.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

References

Submitted references

Protective effect of 7-O-succinyl macrolactin A against intestinal inflammation is mediated through PI3-kinase/Akt/mTOR and NF-κB signaling pathways.

Park S, Regmi SC, Park SY, Lee EK, Chang JH, Ku SK, Kim DH, Kim JA
European journal of pharmacology 2014 Jul 15;735:184-92

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (bs-0608R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 60kD. Observed band size: 60kD.

Sample type

Rat

Other comments

Brain

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Sample type

Rat

Other comments

Brain

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (bs-0608R), Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C and DAPI staining

Sample type

Mouse

Other comments

Heart