LS-C377006

antibody from LSBio

Targeting: VTN VN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C377006

Provider

LSBio

Product name

VTN / Vitronectin Antibody (aa364-478) LS-C377006

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot. All lanes: Vitronectin antibody at 12 ug/ml+HepG2 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 54 kDa. Observed band size: 54 kDa Immunohistochemistry.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: Vitronectin antibody at 12µg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating VTN in NIH/3T3 whole cell lysate Lane 1: Rabbit control IgG instead of VTN Antibody in NIH/3T3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: VTN Antibody (8µg) + NIH/3T3 whole cell lysate (500µg) Lane 3: NIH/3T3 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating VTN in NIH/3T3 whole cell lysate Lane 1: Rabbit control IgG instead of VTN Antibody in NIH/3T3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: VTN Antibody (8µg) + NIH/3T3 whole cell lysate (500µg) Lane 3: NIH/3T3 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating VTN in NIH/3T3 whole cell lysate Lane 1: Rabbit control IgG instead of VTN Antibody in NIH/3T3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: VTN Antibody (8µg) + NIH/3T3 whole cell lysate (500µg) Lane 3: NIH/3T3 whole cell lysate (10µg)

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human small intestine using antibody at 1:100 dilution.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using antibody at 1:100 dilution.