DMAB9590

antibody from Creative Diagnostics

Targeting: CAPZB

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

DMAB9590

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DMAB9590, RRID:AB_2393134

Product name

Anti-CAPZB Monoclonal antibody, clone 5B12.3

Antibody type

Monoclonal

Description

Capping protein (CP), a ubiquitous actin binding protein composed of an alpha and a beta subunit, is important for actin assembly and cell motility. Lower organisms have one gene and one isoform of each subunit. Chickens have two very similar alpha-subunit isoforms. To determine if vertebrates in general contain multiple alpha isoforms and if those alpha isoforms have conserved sequences, we isolated and analyzed alpha subunit cDNA"s in mice and humans. Both mice and humans also have two alpha isoforms. Phylogenetic analysis of the alpha isoform sequences reveals that vertebrates have two highly conserved subfamilies, alpha1 and alpha2. The alpha1 and alpha2 subfamilies are very similar to each other but can be defined and distinguished from each other by a small number of key amino acid residues. In addition, 3" untranslated cDNA sequences are conserved within the isoform subfamilies. To investigate the function of the alpha isoforms, we examined their expression in mouse cells and tissues. Endothelial cells contain only the alpha2 isoform, and erythrocytes contain almost exclusively the alpha1 isoform. Most tissues have both alpha1 and alpha2 isoforms but the ratio of alpha1:alpha2 varies widely. Together, these findings support the hypothesis that the CP alpha isoforms have conserved, unique and essential roles in vertebrates.

Reactivity

Chicken/Avian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

5B12.3

Vial size

1 ea

Storage

-20 °C, Avoid freeze / thaw cycles