GTX25832

antibody from GeneTex

Targeting: HNRNPA1 ALS20, hnRNP-A1, HNRPA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX25832

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25832, RRID:AB_380710

Product name

hnRNP A1 antibody [9H10]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster, Simian

Host

Mouse

References

Submitted references

Targeting RNA foci in iPSC-derived motor neurons from ALS patients with a C9ORF72 repeat expansion.

Sareen D, O'Rourke JG, Meera P, Muhammad AK, Grant S, Simpkinson M, Bell S, Carmona S, Ornelas L, Sahabian A, Gendron T, Petrucelli L, Baughn M, Ravits J, Harms MB, Rigo F, Bennett CF, Otis TS, Svendsen CN, Baloh RH
Science translational medicine 2013 Oct 23;5(208):208ra149

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Interaction with polyglutamine aggregates reveals a Q/N-rich domain in TDP-43.

Fuentealba RA, Udan M, Bell S, Wegorzewska I, Shao J, Diamond MI, Weihl CC, Baloh RH
The Journal of biological chemistry 2010 Aug 20;285(34):26304-14

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. GTX25832). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. COS7 5. MDBK 6. NRK 7. CHO

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 10 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. GTX25832). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjμgate.