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Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAM1124 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD11b/Integrin alpha M Biotinylated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- M1/70
- Vial size
- 250 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6379-93
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6379-93
Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
Journal of virology 2009 Jan;83(2):522-39
Journal of virology 2009 Jan;83(2):522-39
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- CD11b/Integrin alpha M in Mouse Spleno-cytes. CD11b/Integrin alpha M was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin alpha M Biotinylated Monoclonal Antibody (Catalog # BAM1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
