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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- Ab170287 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD14 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human CD14 Antibody, Monoclonal (UCHM1), could be used for Flow and so on. Application: Flow: < 0.5 μg/100w cells Protein Function Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- MEM-15
- Vial size
- 10μg
- Concentration
- Lot by Lot
- Storage
- Shipped at 4°C. Store at 4°C long term (36 months). Upon delivery aliquot.
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Supportive validation
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- Aladdin Scientific (provider)
- Main image
- Experimental details
- CD14 Mouse mAb (Ab170287) - Flow Cytometry Flow Cytometry analysis of A549 cells labelling CD14 (red) with CD14 Mouse mAb (Ab170287) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (Alexa Fluor® 647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
