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Learn10174-1-AP
antibody from Invitrogen Antibodies
Targeting: NKIRAS2
DKFZP434N1526, kappaB-Ras2, KBRAS2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 10174-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NKIRAS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGKSCKVVV CGQASVGKTS ILEQLLYGNH VVGSEMIETQ EDIYVGSIET DRGVREQVRF YDTRGLRDGA ELPRHCFSCT DGYVLVYSTD SRESFQRVEL LKKEIDKSKD KKEVTIVVLG NKCDLQEQRR VDPDVAQHWA KSEKVKLWEV SVADRRSLLE PFVYLASKMT QPQSKSAFPL SRKNKGSGSL DG (1-191 aa encoded by BC007450)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP ( NKIRAS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10174-1-AP (NKIRAS2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 10174-1-AP (NKIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NKIRAS2 (IP:10174-1-AP, 4ug; Detection:10174-1-AP 1:300) with HEK-293 cells lysate 2400 ug.
