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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-88550 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM; Positive Samples: MCF-7, DU145, HL-60, PC-3, Mouse liver, Mouse spleen; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.97 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PSMA3 in extracts of various cell lines using PSMA3 Polyclonal Antibody (Product # PA5-88550) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PSMA3 in extracts of various cell lines using PSMA3 Polyclonal Antibody (Product # PA5-88550) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MCF-7 cells using PSMA3 Polyclonal antibody (Product # PA5-88550). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PSMA3 was performed in MCF-7 cells using PSMA3 Polyclonal Antibody (Product # PA5-88550).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of PSMA3 was performed in 200 µg extracts of HL-60 cells using PSMA3 Polyclonal Antibody (Product # PA5-88550). Western blot was performed from the immunoprecipitate using PSMA3 Polyclonal Antibody.
