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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- MA5-16154 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK beta Monoclonal Antibody (10AG2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Suggested positive control: Daudi, HeLa, NIH-3T3.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10AG2
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Transgenic inhibition of astroglial NF-κB restrains the neuroinflammatory and neurodegenerative outcomes of experimental mouse glaucoma.
Yang X, Zeng Q, Barış M, Tezel G
Journal of neuroinflammation 2020 Aug 28;17(1):252
Journal of neuroinflammation 2020 Aug 28;17(1):252
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKKbeta on Lysate from A) human Daudi, B) HeLa, and C) mouse NIH 3T3 cells using a IKK-beta monoclonal antibody (Product # MA5-16154) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK beta in A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate. Samples were incubated in IKK beta monoclonal antibody (Product # MA5-16154) using a dilution of 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK beta in 1.0 mg/mL HeLa lysate. Samples were incubated in IKK beta monoclonal (Product # MA5-16154). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IKK beta in formalin-fixed paraffin-embedded tissue section of human placenta. Samples were incubated in IKK beta monoclonal antibody (Product # MA5-16154) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis using IKK-beta monoclonal antibody (Product # MA5-16154). HEK293 cells were probed with 0.5 µg of IKK-beta monoclonal antibody (Product # MA5-16154) (red) and 0.5 µg of isotype control antibody. An mouse IgG1 PE conjugated secondary.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IKK beta in Jurkat cells. Samples were incubated in IKK beta monoclonal antibody (Product # MA5-16154). Antibody (conjugated to PE) (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IKK beta in HeLa cells. Samples were incubated in IKK beta monoclonal antibody (Product # MA5-16154) using a dilution of 1 µg of antibody in 100 µL of staining buffer with cells incubated for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IKK beta in HEK 293 cells. Samples were incubated in IKK beta monoclonal antibody (Product # MA5-16154) using a dilution of 0.1 µg. Green histogram represents the isotype control , red represents the IKKbeta antibody (conjugated toAlexa Fluor (R) 488).
