Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-47003 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human IL-23 p19 and recombinant mouse IL-23 p19 was observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47003) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-12/IL-23 p40 in Hs 294T human melanoma cell line. Samples were incubated in IL-12/IL-23 p40 polyclonal antibody (Product # PA5-47003) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for IL‚12/IL‚23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-12/IL-23 p40 is shown by the decrease in mean OD (measure of IL-17 secretion) with increasing concentrations of IL-12/IL-23 p40 Polyclonal Antibody (PA5-47003).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-12/IL-23 p40 in mouse splenocytes. Samples were incubated in IL-12/IL-23 p40 polyclonal antibody (Product # PA5-47003). Recombinant Human IL‚23 induces IL-17 secretion in the mouse splenocytes in a dose‚dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL‚23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti‚Human IL‚12/IL‚23 p40 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.004-0.02 μg/mL.