Antibody data
- Antibody Data
- Antigen structure
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- Product number
- 13-7129-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Reported: The C8.6 antibody has been reported for use in ELISA. Applications Tested: The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the purified B-T21 (13-7128) antibody for capture and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5 - 2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p40 monomer in combination with the purified C8.3 (14-7127) antibody for capture and recombinant human IL-12 p40 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10000 pg/mL - 80 pg/mL should be included in each ELISA plate. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- C8.6
- Vial size
- 500 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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