Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 511202 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#511202, RRID:AB_416324
- Product name
- Purified anti-human IL-23 (p19)
- Antibody type
- Monoclonal
- Antigen
- Human recombinant p40 and p19 with elastin linker
- Description
- The HLT2736 clone recognizes human IL-23, a heterodimeric cytokine composed of the p40 subunit shared with IL-12 and a specific p19 subunit. This antibody recognizes heterodimeric IL-23, but does not react with the p40 subunit alone or recombinant IL-12(p70) when used as a capture antibody in ELISA. IL-23 is secreted by activated dendritic cells and macrophages and has been shown to enhance IFN-γ secretion by memory (CD45RO) T cells in an IL-2 dependent manner. IL-23 may also induce unique Th subset (designated ThIL-17) that secretes the cytokines IL-17, IL-6, TNF and low levels of IFN-γ. IL-23 has been shown to promote immunity to mycobacteria and has been shown to be upregulated in response to some viral infections and in psoriatic lesions. IL-23 binds to the IL-23 receptor comprised of a β1 subunit shared with the IL-12 receptor and IL-23-specific subunit. This antibody has been shown to be useful for Western blotting, immunohistochemistry using formalin-fixed, paraffin embedded tissues with (catalyzed signal amplification), and immunoprecipitation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4° C.
Submitted references Proteasome inhibitors prevent caspase-1-mediated disease in rodents challenged with anthrax lethal toxin.
Systemic and local interleukin-17 and linked cytokines associated with Sjögren's syndrome immunopathogenesis.
The involvement of IL-23 and the Th17 pathway in periodontitis.
Muehlbauer SM, Lima H Jr, Goldman DL, Jacobson LS, Rivera J, Goldberg MF, Palladino MA, Casadevall A, Brojatsch J
The American journal of pathology 2010 Aug;177(2):735-43
The American journal of pathology 2010 Aug;177(2):735-43
Systemic and local interleukin-17 and linked cytokines associated with Sjögren's syndrome immunopathogenesis.
Katsifis GE, Rekka S, Moutsopoulos NM, Pillemer S, Wahl SM
The American journal of pathology 2009 Sep;175(3):1167-77
The American journal of pathology 2009 Sep;175(3):1167-77
The involvement of IL-23 and the Th17 pathway in periodontitis.
Ohyama H, Kato-Kogoe N, Kuhara A, Nishimura F, Nakasho K, Yamanegi K, Yamada N, Hata M, Yamane J, Terada N
Journal of dental research 2009 Jul;88(7):633-8
Journal of dental research 2009 Jul;88(7):633-8
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Supportive validation
- Submitted by
- BioLegend (provider)
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- Experimental details
- Linker-free, recombinant human IL-23 was resolved by electrophoresis, transferred to nitrocellulose and probed with the anti-IL23 p19 specific antibody HLT2736 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. The HLT2736 recognizes the p19-specific subunit of human IL-23. This antibody will also recognize human IL-23 (p19 +p40 subunits) in recombinant IL-23 containing a linker moiety. For IHC data using this antibody, please click on "Product Data Sheet PDF".
- Conjugate
- Unconjugated