MA1-46412

antibody from Invitrogen Antibodies

Targeting: HAP1 HAP2, hHLP1, HIP5, HLP

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-46412

Provider

Invitrogen Antibodies

Product name

HAP1 Monoclonal Antibody (1B6)

Antibody type

Monoclonal

Antigen

Other

Description

In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B. Suggested positive control: rat brain.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1B6

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.

Cason SE, Carman PJ, Van Duyne C, Goldsmith J, Dominguez R, Holzbaur ELF
The Journal of cell biology 2021 Jul 5;220(7)

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Mutant huntingtin impairs PNKP and ATXN3, disrupting DNA repair and transcription.

Gao R, Chakraborty A, Geater C, Pradhan S, Gordon KL, Snowden J, Yuan S, Dickey AS, Choudhary S, Ashizawa T, Ellerby LM, La Spada AR, Thompson LM, Hazra TK, Sarkar PS
eLife 2019 Apr 17;8

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Huntingtin-associated protein 1: Eutherian adaptation from a TRAK-like protein, conserved gene promoter elements, and localization in the human intestine.

Lumsden AL, Young RL, Pezos N, Keating DJ
BMC evolutionary biology 2016 Oct 13;16(1):214

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Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons.

Yang GZ, Yang M, Lim Y, Lu JJ, Wang TH, Qi JG, Zhong JH, Zhou XF
Journal of neurochemistry 2012 Sep;122(5):1010-22

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of HAP1 in mouse brain lysate using Product # MA1-46412.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAP1 in Lane 1: mouse brain lysate and Lane 2: human brain lysate. Samples were incubated in HAP1 monoclonal antibody (Product # MA1-46412).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HAP1 in mouse brain lysate. Sample was incubated in HAP1 monoclonal antibody (Product # MA1-46412).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of HAP1 in rat hypothalamus. Samples were incubated in HAP1 monoclonal antibody (Product # MA1-46412).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of HAP1 in 1 x 10^6 MCF-7 cells. Samples were incubated in HAP1 monoclonal antibody (Product # MA1-46412) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 HAP1 and 5-HT localization in villi from healthy human duodenum. ( a-e ) are related images highlighting an example of cells individually positive for HAP1 or 5-HT. Images ( a-d ) are the same field of view (enlarged from e ), showing HAP1 alone ( a ), 5-HT alone ( b ), merged ( c ), merged with DAPI nuclei stain ( d ), with lumen to the left. Image e shows HAP1, 5-HT and DAPI staining in the wider context of the villus. Images ( f-j ) highlight a cell immunopositive for both HAP1 and 5-HT. Lumen is to the top right in ( f-i ). Epifluorescence images taken with 40x magnification. Scale bar = 20 mum