LS-C746727

antibody from LSBio

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C746727

Provider

LSBio

Product name

CD44 Antibody LS-C746727

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat lung using CD44 antibody at dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using CD44 antibody at dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human trachea using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using CD44 antibody at dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat lung using CD44 antibody at dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using CD44 antibody at dilution of 1:100 (40x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human trachea using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat lung using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat spleen using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using CD44 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen using CD44 antibody at dilution of 1:100 (40x lens).