103001

antibody from BioLegend

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

103001

Provider

BioLegend

Proper citation

BioLegend Cat#103001, RRID:AB_312952

Product name

Purified anti-mouse/human CD44

Antibody type

Monoclonal

Antigen

Dexamethasone-induced myeloid leukemia M1 cells

Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Reactivity

Human, Mouse, Bovine, Canine, Feline, Horse, Porcine, Simian

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Cucurbitacin I suppressed stem-like property and enhanced radiation-induced apoptosis in head and neck squamous carcinoma--derived CD44(+)ALDH1(+) cells.

Chen YW, Chen KH, Huang PI, Chen YC, Chiou GY, Lo WL, Tseng LM, Hsu HS, Chang KW, Chiou SH
Molecular cancer therapeutics 2010 Nov;9(11):2879-92

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Identification of cancer stem cells in a Tax-transgenic (Tax-Tg) mouse model of adult T-cell leukemia/lymphoma.

Yamazaki J, Mizukami T, Takizawa K, Kuramitsu M, Momose H, Masumi A, Ami Y, Hasegawa H, Hall WW, Tsujimoto H, Hamaguchi I, Yamaguchi K
Blood 2009 Sep 24;114(13):2709-20

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Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.

Kmieciak M, Gowda M, Graham L, Godder K, Bear HD, Marincola FM, Manjili MH
Journal of translational medicine 2009 Oct 22;7:89

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Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos.

Wang XY, Lan Y, He WY, Zhang L, Yao HY, Hou CM, Tong Y, Liu YL, Yang G, Liu XD, Yang X, Liu B, Mao N
Blood 2008 Feb 15;111(4):2436-43

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Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses.

Kenna TJ, Thomas R, Steptoe RJ
Blood 2008 Feb 15;111(4):2091-100

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The aged thymus shows normal recruitment of lymphohematopoietic progenitors but has defects in thymic epithelial cells.

Gui J, Zhu X, Dohkan J, Cheng L, Barnes PF, Su DM
International immunology 2007 Oct;19(10):1201-11

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ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.

Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH
Cancer research 2005 May 15;65(10):4320-33

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The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes.

Zhang N, He YW
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):6967-73

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 mouse splenocytes stained with purified IM7, followed by anti-rat IgG FITC

Conjugate

Unconjugated